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Clone Development

We can provide services from gene synthesis to characterized cell banks. We undertake milestone based approach with definite deliverables from gene cloning to selection of final clonal cell line.

Clone development services for microbial and mammalian cells include:

Microbial

  • Gene synthesis, codon optimization and cloning into suitable vectors.
  • Multiple hosts screening for identification of suitable host strain
  • Expression & productivity analysis
  • Stability analysis of selected clones
  • Cell banking and characterization

Mammalian

  • Synthesis of codon optimized genes.
  • Cloning in suitable expression vectors
  • Confirmation of protein expression by initial transient transfection trials
  • Transfection and stable pool generation using suitable selection and amplification
  • Clonal selection by limiting dilution and screening of clones
  • Productivity and stability analysis of selected clones



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