Putting Science to Work
ADME-DMPK

ADME/DMPK

ADME & DMPK Assays & Analysis

We provide a broad portfolio of in vitro assays that enable evaluation of critical ADMET parameters for pharmaceutical drug discovery.

ADMET Assays
  • Physico-chemical properties - aqueous solubility, pH stability, Lipophilicity (Log D) and Log P
  • Absorption – PAMPA, CaCo-2 and MDCK, Efflux (CaCo-2)
  • Metabolism & stability - Liver microsomes (%PCR and Clint), plasma and serum stability, CYP450 inhibition includes inhibition screening (Fluorescence based/LC-MS/MS based) and time - dependent inhibition (TDI), CYP450 phenotyping and metabolite identification (LC-MS/MS) (in vitro & in vivo)
  • Distribution - plasma protein binding, brain protein binding and blood-plasma distribution (Ce/Cp)
  • Cytotoxicity - cell-based assays in human cell- lines
PK Studies

Early stage PK evaluation of NCE's in rodents:

  • Discrete and cassette dosing
  • Tissue distribution
  • Brain-plasma distribution
  • Entero-hepatic recirculation (EHC)
  • Determination of fundamental PK parameters (Tmax, Cmax, AUC, clearance, oral bioavailability, volume of distribution, etc.)
Bioanalysis

Development of bioanalytical methods to support bioavailability and pharmacokinetics studies.

  • HPLC -UV and LC-MS/MS based methods
  • Development of efficient extraction procedures (protein precipitation, liquid- liquid extraction, solid-phase extraction)
  • Development of analytical methods in presence of different matrices

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