Putting Science to Work
clone-development

Clone Development

We can provide services from gene synthesis to characterized cell banks. We undertake a milestone based approach with definite deliverables from gene cloning to selection of final clonal cell line.

Clone development services for microbial and mammalian cells include:

Microbial
  • Gene synthesis, codon optimization and cloning into suitable vectors
  • Multiple hosts screening for identification of suitable host strain
  • Expression & productivity analysis
  • Stability analysis of selected clones
  • Cell banking and characterization
Mammalian
  • Synthesis of codon optimized genes.
  • Cloning in suitable expression vectors
  • Confirmation of protein expression by initial transient transfection trials
  • Transfection and stable pool generation using suitable selection and amplification
  • Clonal selection by limiting dilution and screening of clones
  • Productivity and stability analysis of selected clones

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