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Gene Expression
High levels of protein expression are critical for efficient and economical purification of active recombinant proteins. We offer a number of expression systems to meet specific goals, ranging from expression of untagged/tagged proteins, receptor/ligand, GPCRs to production of biopharmaceuticals for clinical use.
Gene expression studies are initiated by carrying out a preliminary feasibility study wherein an appropriate host system is chosen for expression of the selected gene(s). Thereafter, various expression conditions (codon usage, choice of promoter, growth and induction conditions, media composition, etc.) are employed to select the optimal conditions for the gene expression. Host systems offered for expression studies are Bacteria, Yeast, Insect and Mammalian cell lines.
Gene Expression in Bacteria:
- Bacterial hosts such as E.coli, Bacillus, Streptomyces
- Screening in E. coli to identify optimal conditions for expression.
- Small scale expression of recombinant protein in selected E. coli strain and under optimal conditions
- Scale up: 2 - 10 litres (access to 20 - 225 litres pilot scale)
Gene Expression - Insect and Mammalian Cell Lines:
- Expression in insect cell lines (Baculovirus expression system)
- Insect Cell Lines : Sf9, Sf21, High Five
- Generation of high titer recombinant virus
- MOI studies
- Small-scale expression studies
- Large scale expression for protein purification (Wave bag systems)
- Expression in mammalian cell lines
- Mammalian Cell lines: CHO-K1, HEK 293, 3T3, MCF, etc.
- Bulk transient transfection for purification of secreted proteins
- Generation of stable cell lines expressing recombinant proteins
- Cell line initiation through transfection and selection
- Clonal selection
- Amplification
- Analysis of expression by various biochemical and functional method
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