Gene Library Construction & Screening
We provide library construction and screening services of gDNA, cDNA and BAC libraries. Libraries can be provided by clients or constructed at Syngene based on client requirements / specification. Screening is carried out by radioactive hybridization or functional activity. Steps involved in screening include:
- Primary screening
- Secondary screening
- Tertiary screening
Analysis of putative clones obtained from screening involves:
- Purification of single colony or plaque
- Further analysis of the clone by Primer walking and Contig assembly of sequences
- Identification of putative ORFs
Cloning of full length ORF
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