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Gene Library Construction & Screening
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  Gene Library Construction & Screening

We provide library construction and screening services of gDNA, cDNA and BAC libraries. Libraries can be provided by clients or constructed at Syngene based on client requirements / specification. Screening is carried out by radioactive hybridization or functional activity. Steps involved in screening include:

  - Primary screening
  - Secondary screening
  - Tertiary screening

Analysis of putative clones obtained from screening involves:

  - Purification of single colony or plaque

  - Further analysis of the clone by Primer walking and Contig     assembly of sequences

  - Identification of putative ORFs
    Cloning of full length ORF

 
     
Gene Library
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INSUGEN® | Arogya Raksha Yojana | Biocon Foundation | ABLE | Karnataka Vision Group on Biotechnology | Karolinska Institute
 
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