Cell Line Development & Analysis
Syngene can generate homogenous stable clones in different cellular backgrounds including very hard-to-transfect cell lines and primary cells, for expressing genes of interest at high levels for reagent generation, assay development and biotherapeutic development.
We specialize in the over-expression of tagged and untagged proteins; G-protein coupled receptors receptor/ ligand, Ion channels, micro RNA target genes, transcription factors and reporter genes. The activities include cell transfections for generation of recombinant cell lines, optimization of transfection, serum free adaptation, media optimization, cell line characterization, monoclonal antibody production and research cell banking.
Cell Culture Capacity
- Adherent: 96 well plate to Cell stacks
- Suspension: 30ml to 3 L Spinners
- Scale of expression: 2 - 10 L lab scale, 1-25 L Wave bioreactor and 50 - 300 L pilot scale
Cell Line Characterization
- Real time PCR (96 and 384 well format)
- Northern blot and Western blot
- Flow cytometry (FACS)
- Functional cell based assays in 384/96 formats
Cell banking plays an important role in establishing cell stocks. In case of microbial contamination of routinely used cells, cryopreserved cells assure a back-up as well as continuous supply. Our services include expansion of cells for cryopreservation, viability testing, preparation of Research Cell Bank (RCB), and sterility and Mycoplasma testing.