Putting Science to Work

Screening & Assay Biology

We encourage our clients to explore with us the advantages of our assay services and leverage our proven technologies to their advantage. Our screening capabilities span from biochemical to cell-based functional assays as given below.

Assay Development and Validation
  • Adaptation for higher plate format (384 and above)
  • Assay compatibility with DMSO
  • Z- factor
  • Validation with reference compounds
  • Rank order potency and IC50 determination
  • Kinetics (Type of inhibition, inhibition constant determination)
  • Homogeneous assays for rapid screening
Biochemical Assays
  • Types of assays are Spectrophotometric, Luminometric, Fluorimetric, and Radiometric
  • Detection methods are Fluorescence (FI , FP, FRET, TR - FRET (HTRF®, LanthaScreen®, LANCE®, DELFIA ®, AlphaScreen ®)), Luminescence (Reporter gene assays (Luciferase, GFP, ?- galactosidase)), Absorbance (UV/ VIS), Radiometric (Fliter-binding and SPA)
  • Assay targets includes Enzymes (Kinases, Proteases, Oxygenases), Phosphatases, Polymerases, GPCRs/ other membrane proteins, Transporters, Nuclear receptors and Protein-protein interactions
Protein Characterization, Analysis and Quality Control

A myriad of techniques are employed to analyse each batch of protein produced that ensures strict quality control and minimal batch to batch variation:

  • Electrophoresis (SDS-PAGE, native-PAGE)
  • Capillary gel Electrophoresis (CGE)
  • DOT/Western blot
  • Imaging Capillary Electrophoresis (iCE)
  • Mass spectrometry (ESI-MS/MS, MALDI-TOF/TOF)
  • Peptide mass finder printing
  • Reverse Phase-HPLC
  • Size Exclusion HPLC
  • Glycoform analysis
  • Endotoxin level detection
  • Biological activity assays
Cell - Based Assays
  • Proliferation (Tetrazolium based/ Alamar blue/ [3H]-Thymidine incorporation)
  • Death (Cytotoxicity)
    • Apoptosis (Caspase 3 & 8, DNA laddering)
    • Necrosis (Calcein AM, Trypan blue exclusion, ATP depletion, LDH release)
  • Receptor binding/ uptake (SPA in flash plates)
  • Metabolism and protein turnover (labelled amino acid incorporation in SPA)
  • Signalling & second messenger generation (cAMP/cGMP/Ca2+efflux)
  • Phosphorylation (CELISA)
  • Reporter gene (Luminescence, GFP and ß-Gal)
  • Chemokine/ Cytokine release (ELISA)
  • Cis/trans activation (NFkb activation)
  • Ion channel (patch clamp assays)
  • GPCRs (Functional and whole-cell ligand binding assays)

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