The in vitro mammalian cell gene mutation test can be used to detect gene mutations induced by chemical substances.
| Test model | CHO K1 |
| Assay controls | Vehicle control: Aqueous or organic solvents Positive controls: Ethyl methane sulphonate 7,12-Dimethylbenzanthracene |
| Exposure time | 3 – 6 hours 20 – 22 hours |
| Test item quantity | 10 mm, 2 mg/mL or 2 μL/mL |
| End point | Number of cells plated in selective and non-selective medium |
| Data delivery | Relative survival and mutant frequency |
| Timeline | 10 – 11 weeks |