The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells.
| Test model | Chinese hamster ovary (CHO), human peripheral blood lymphocytes |
| Assay controls | Negative control: Aqueous and organic solvents Positive control – Methyl methane sulphonate/ ethyl Methane sulphonate, cyclophosphamide monohydrate and vinblastine |
| Exposure time | Short duration: With and without metabolic Activation, 3 – 6 hrs Prolonged duration treatment: Without metabolic activation, 20 – 21 hrs |
| Test item quantity | 10 mM, 2 mg/mL or 2 μl/mL |
| End point | Micronucleus evaluation in 2000 binucleate cells |
| Data delivery | Percentage of micronuclei and replication index |
| Timeline | 10 – 12 Weeks |
