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In Vitro 3T3 NRU Phototoxicity Test

In Vitro 3T3 NRU Phototoxicity Test

The in vitro 3T3 Neutral Red Uptake (NRU) phototoxicity test is used to identify the phototoxic potential of a test chemical activated by exposure to light. The test evaluates photo-cytotoxicity by the relative reduction in viability of cells exposed to the test chemical in the presence versus absence of light.

Test Model Balb/c 3T3 cells
Replicates Six per group (preliminary and confirmatory study)
Assay controls Negative control: Aqueous or organic solvents
Positive control: Chlorpromazine (CPZ)
Eight different test item concentrations
Exposure time 5 J/CM2 for 50 minutes
Test item quantity 1.0 gram
End point The ability of viable cells to incorporate and bind neutral
red (NR) in the presence and absence of light
Data delivery IC50 calculation and photo-irritation factor
Timeline 3 – 4 weeks

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