Molecular Biology at Syngene provides a wide range of services in the area of recombinant DNA technology, library construction and screening, strain engineering and gene expression analysis.
Recombinant DNA Technology & Engineering
We provide Recombinant DNA Engineering services in the following areas:
- Gene cloning & vector construction
Syngene has expertise in gene cloning by various methods such as high throughput recombination-based cloning — gateway technology, infusion cloning, Gibson assembly, de novo cloning (PCR / degenerate PCR/RT-PCR/homology-based PCR, etc.), synthetic genes, oligo — linker cloning, sub-cloning, and RACE. In addition to gene cloning, vector construction can also be carried out for generating tailor made vectors for specific purposes. - V-gene cloning
Antibody light and heavy chain gene cloning by RACE or degenerate primer methods from hybridoma. - Mutagenesis
Types of mutations which we do are point mutation, insertion, deletion or replacement. Each mutagenesis is customized to meet specific client requirements such as random mutagenesis (error-prone PCR) and site-directed mutagenesis (Dpn-I restriction enzyme based, splicing by overlapping extension (SOE) PCR and megaprimer-based). - Genome walking
We have expertise in carrying out genome walking to identify unknown sequences flanking the known gene sequences such as conventional (bacterial/phage library screening) and PCR-based methods.
Gene Library Construction & Screening
We provide the following gene library construction and screening services based on client requirements/ specifications:
- Library construction
We have expertise in gDNA libraries, cDNA libraries, BAC libraries, phage libraries, yeast libraries and cosmid. - Screening
We provide radioactive hybridization and functional activity.
Strain Engineering
We have experience in engineering bacterial and yeast host strains for the improved production of recombinant products and to introduce new catabolic activities.
- Strain modification methods
We provide site directed mutagenesis, gene knock out and insertional inactivation. - Analysis of engineered strains
We have expertise in molecular methods, microbiological methods and productivity analysis.
Gene Expression Analysis
Expression of a gene at the transcriptional and translational levels in a host system is very important in studying the functional gene product. scientists at Syngene routinely carry out the following gene expression analysis:
- Misfit for gene expression analysis
- Real-time PCR
- ELISA
- Flow cytometry
- Western blotting
- Mass spectrometry
- RNA sequencing
Infrastructure
Syngene has a sophisticated biology facility with high-end instrumentation. We have an experienced team with proven track record of supporting discovery and development programs under biology capabilities.
Some of the key instruments are listed below:
Molecular Biology
- Automated DNA sequencer- ABI 3730 XL, ABI 3130XL
- Thermal cyclers
- Real-time PCR instruments (ABI 96 well & 384 well formats)
- Pulsed field electrophoresis
- Gene pulser
- Gel documentation systems
- Nano drop spectrophotometer
Microbiology
- Benchtop fermenters
- Biosafety cabinets- BSL I & II
- Incubators and incubator shakers
- Vitek 2 compact
