• Small Molecules
      • Discovery
    • Large Molecules
    • Other Drug Modalities
    • SynVent Integrated Drug Discovery
      • SynVent Integrated Drug Discovery

        SynVent is Syngene’s platform for fully integrated therapeutic discovery and development across large and small molecules. 

    • Industries
    • Emerging Biopharma
      • Emerging Biopharma

        Emerging biopharma work at the forefront of science, often venturing into disease areas where little or no real-world data exists to work with or regulatory frameworks to work within.

    • Dedicated Centers
      • Dedicated Centers

        Our Dedicated Centers offer dedicated multi-disciplinary scientific teams, support personnel, and a tailormade ring-fenced and fire-walled infrastructure as per client specifications to support their  R&D  goals

    • Center for Advanced Protein Studies (CAPS)
      • Center for Advanced Protein Studies (CAPS)

        Centre for Advanced Protein Studies [CAPS] is a state-of-the-art advanced national facility located in the Syngene campus, Bangalore.

  • Careers

Assay Biology & Screening

Syngene’s assay biology and screening team brings together world-class scientists, state-of-the-art infrastructure, streamlined processes from sample submission to data transfer, and strong quality management to deliver a wide range of assays and screens.

Our assay team develops, validates and screens compounds, with miniaturization of assays achieved by using 384-well plates, which can be further miniaturized to 1536-well formats. We have experience with small and large molecules, peptides, PROTACs and oligonucleotides. Our assays are validated using tool compounds with QC established (signal: noise ratio; Z’-prime; uniformity; reproducibility). Qualification of assays for biosimilars is followed by bio-comparability testing of drug product and RLD meant for regulatory submission purposes.


Biochemical assays

  • High throughput assays meant for different target classes including kinases, non-kinases (proteases, phosphatases, dehydrogenases etc.), GPCRs (binding), nuclear receptors, ion channels (binding) and protein-protein interactions. Detection methods include fluorescence, fluorescence polarization (FP), TR-FRET, (HTRF®, LanthaScreen®, LANCE®, DELFIA ®, AlphaScreen ®)), luminescence and radiometric (filter-binding and SPA)
  • Biophysical assays for Kd determination for small and large molecules using SPR (BiaCore), Octet, DSF, and MST platforms.

Cell – based assays

  • Cell based assays for endpoints of physiological relevance- Immuno-oncology, cancer metabolism, metabolic disorders, NASH, Fibrosis, Autoimmune disorders, CNS, Dermatology, Orphan diseases
  • Target specific assays for GPCRs (FLIPR, cAMP release); ion channels (membrane potential and thallium flux); transporters (radioactive); enzymes (target phosphorylation)
  • Cell proliferation and cytotoxicity assays in high throughput formats in 2D and 3D culture setting
  • 120+ cancer cell lines available for testing purpose
  • High Content Imaging based assays
  • Live cell imaging-based assays
  • Experience with primary cells, PBMCs, immune cells, and iPSCs

Mechanistic Assays

  • High throughput Gene and protein expression studies in cellular and tissue samples from animals and humans
  • Ex vivo studies for target engagement using different tissue samples
  • Biomarker characterization

Infrastructure

Syngene has a sophisticated biology facility with high-end instrumentation. We have an experienced team with proven track record of supporting discovery and development programs under biology capabilities.

Some of the key instruments are listed below:

Assay Biology / Screening

Liquid Handling Systems/Pipetting stations

  • Echo (Acoustic Dispensing)
  • Integra ViaFlo
  • Freedom Evo 150 (Tecan)
  • Multidrop Combi (Thermo)
  • 96 well plate washers

Multimode plate readers with stackers

  • TopCount Nxt HTS (Perkin Elmer)
  • Spectramax PLUS (Molecular Devices)
  • EnVision Multilabel reader (Perkin Elmer)
  • SPARK (Tecan)
  • FLIPR
  • InCell Analyzer
  • WES
  • QPCR
  • Incucyte
  • Multiplex

Bioanalytical

  • HPLCs (Agilent 1200, Shimaduzu Prominence UFLC)
  • LC-MS/MS (API2000, 3200 and 4000)
  • TurboVap LV
  • API- 2000, 3200, 4000, 4000-Q-Trap (Lightsight Software)

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