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      • Discovery
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        SynVent is Syngene’s platform for fully integrated therapeutic discovery and development across large and small molecules. 

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        Emerging biopharma work at the forefront of science, often venturing into disease areas where little or no real-world data exists to work with or regulatory frameworks to work within.

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      • Dedicated Centers

        Our Dedicated Centers offer dedicated multi-disciplinary scientific teams, support personnel, and a tailormade ring-fenced and fire-walled infrastructure as per client specifications to support their  R&D  goals

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      • Center for Advanced Protein Studies (CAPS)

        Centre for Advanced Protein Studies [CAPS] is a state-of-the-art advanced national facility located in the Syngene campus, Bangalore.

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Assay Biology & Screening

Assay Biology & Screening

Syngene’s assay biology and screening team brings together world-class scientists, state-of-the-art infrastructure, streamlined processes from sample submission to data transfer, and strong quality management to deliver a wide range of assays and screens.

Our assay team develops, validates and screens compounds, with miniaturization of assays achieved by using 384-well plates, which can be further miniaturized to 1536-well formats. We have experience with small molecules and biologics, peptides, PROTACs and oligonucleotides. Our assays are validated using tool compounds with QC established (signal: noise ratio; Z’-prime; uniformity; reproducibility). Qualification of assays for biosimilars is followed by bio-comparability testing of drug product and RLD meant for regulatory submission purposes.

Biochemical assays

  • High throughput assays meant for different target classes including kinases, non-kinases (proteases, phosphatases, dehydrogenases etc.), GPCRs (binding), nuclear receptors, ion channels (binding) and protein-protein interactions. Detection methods include fluorescence, fluorescence polarization (FP), TR-FRET, (HTRF®, LanthaScreen®, LANCE®, DELFIA ®, AlphaScreen ®)), luminescence and radiometric (filter-binding and SPA)
  • Biophysical assays for Kd determination for small molecules and biologics using SPR (BiaCore), Octet, DSF, and MST platforms.

Cell-based assays

  • Cell based assays for endpoints of physiological relevance—Immuno-oncology, cancer metabolism, metabolic disorders, NASH, fibrosis, autoimmune disorders, CNS, dermatology, orphan diseases
  • Target specific assays for GPCRs (FLIPR, cAMP release); ion channels (membrane potential and thallium flux); transporters (radioactive); enzymes (target phosphorylation)
  • Cell proliferation and cytotoxicity assays in high throughput formats in 2D and 3D culture setting
  • 120+ cancer cell lines available for testing purpose
  • High content imaging-based assays
  • Live cell imaging-based assays
  • Experience with primary cells, PBMCs, immune cells, and iPSCs

Mechanistic assays

  • High throughput gene and protein expression studies in cellular and tissue samples from animals and humans
  • Ex vivo studies for target engagement using different tissue samples
  • Biomarker characterization


Syngene has a sophisticated biology facility with high-end instrumentation. We have an experienced team with proven track record of supporting discovery and development programs under biology capabilities.

Some of the key instruments are listed below:

Assay biology/screening

Liquid handling systems/pipetting stations

  • Echo (Acoustic Dispensing)
  • Integra ViaFlo
  • Freedom Evo 150 (Tecan)
  • Multidrop combi (Thermo)
  • 96-well plate washers

Multimode plate readers with stackers

  • TopCount Nxt HTS (Perkin Elmer)
  • Spectramax PLUS (Molecular Devices)
  • EnVision Multilabel reader (Perkin Elmer)
  • SPARK (Tecan)
  • InCell analyzer
  • WES
  • QPCR
  • Incucyte
  • Multiplex


  • HPLCs (Agilent 1200, Shimaduzu Prominence UFLC)
  • LC-MS/MS (API2000, 3200 and 4000)
  • TurboVap LV
  • API- 2000, 3200, 4000, 4000-Q-Trap (Lightsight software)

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